Endoret inhibits apoptotic signaling pathway in Ab-stimulated neuronal cultures.

A. 7.5 and 10% Endoret treatment protects against Ab_1-42 (10 µM)-induced cell death in neuronal cell culture assayed with a cell death ELISA. B. Fluorescent images of living cells (green) and dead cells (red) in neuronal cultures in control condition (B27), and after addition of Ab_1-42 (10 µM), and 7.5 and 10% Endoret. C. Quantification of Ab_1-42-induced cell death after 48 h in vitro. Representative blots and quantitative analysis of (D) caspase-3, and (E) HSP-70 expression in neuronal cultures treated with Aβ₄₂ (10 µM) and 7.5 and 10% Endoret. One representative experiment is shown (n = 3 experiments). Data show mean ± SEM; *p<0.05 and **p<0.01 vs control culture. #p<0.05 and # #p<0.01 vs Aβ₄₂ -treated culture.