Endogenous GARP levels in Tregs are reduced after transfection of miR-181a, miR-142-3p and miR-185 mimics.
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Polyclonal CD4+CD25+CD127lo populations were purified from human PBMCs and amplified in vitro. Amplified cells were electroporated with the indicated miRNA mimics and stimulated 6 hours later with anti-CD3/CD28 antibodies. Cell lysates were collected 24 hours later and analyzed by WB with anti-GARP and anti-β-ACTIN antibodies.