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Endogenous GARP levels in Tregs are reduced after transfection of miR-181a, miR-142-3p and miR-185 mimics.

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posted on 30.09.2013, 03:04 by Emilie Gauthy, Julia Cuende, Julie Stockis, Caroline Huygens, Bernard Lethé, Jean-François Collet, Guido Bommer, Pierre G. Coulie, Sophie Lucas

Polyclonal CD4+CD25+CD127lo populations were purified from human PBMCs and amplified in vitro. Amplified cells were electroporated with the indicated miRNA mimics and stimulated 6 hours later with anti-CD3/CD28 antibodies. Cell lysates were collected 24 hours later and analyzed by WB with anti-GARP and anti-β-ACTIN antibodies.

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