Endogenous AIPL1 co-localizes with ciliary markers and EB1 in human retinal cryosections.
A: Immunohistochemical localization of AIPL1 (green) and centrin-3 (red) in unfixed human retinal cryosections. Nuclei are labelled with DAPI (blue). The labelling on the DIC (differential interference contrast) image is: ganglion cell layer (GCL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); photoreceptor inner segments (IS) and photoreceptor outer segments (OS). B: Zoomed image of the immunohistochemical localization of AIPL1 (green) and centrin-3 (red) in photoreceptor cells. The white squares demarcate a further zoomed area (left hand column) highlighting the enrichment of AIPL1 (green) and centrin-3 in the cilia (white arrowheads). Nuclei are labelled with DAPI (blue). Inner segments (IS); connecting cilia (cc); outer segments (OS). Scale bar: 10 μm. C: Immunogold labelling of EB1 and AIPL1 in human retinal cryosections. 80 nm human retinal cryosections were double-labelled with antibodies against EB1 (PAG 10 nm, small arrow) and AIPL1 (PAG 15 nm, large arrowhead). Inner segments (IS); outer segments (OS); connecting cilium (cc); basal body (bb); microtubules (mt). Scale bar: 200 nm.