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Encapsulation response of G. mellonella was decreased during Candida pathogenesis.

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posted on 15.11.2013, 03:05 by Nadja Rodrigues de Melo, Ahmed Abdrahman, Carolyn Greig, Krishnendu Mukherjee, Catherine Thornton, Norman A. Ratcliffe, Andreas Vilcinskas, Tariq M. Butt

Melanization of the implants was quantified to determine if encapsulation was affected during Candida pathogenesis after implants were introduced into the left pro-leg of larvae 24 h post- infection. Two hours after insertion of implants, during C. albicans pathogenesis (n = 5, triplicated), the degree of the melanization was quantified using Image Pro software by first measuring the coloration (gray value) of all areas of each implant, and then comparing these values with intact implants (not inserted). Decreased encapsulation responses were observed in larvae infected with Candida with or without pre treatment with MYR compared with PBS injected larvae (Figure A, *p<0.05). Plastic implants inserted into C. albicans-infected Galleria larvae showed markedly reduced melanization illustrated in the Figure C compared with PBS control insects (Figure B).


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