Embryonic developmental expression of Kif5b in mouse kidney.
(A-D) Immunolocalization of Kif5b protein in the fetal mouse kidney at embryonic days (E) 12.5, 16.5. (A, B) Kif5b was not detectable in the E12.5 fetal kidney. (C, D) At E16.5, Kif5b was highly expressed in the ureteric bud (UB) and tubules (T). Images are representative of kidney tissue sections from three mice. (E) Quantitative real-time RT-PCR analysis showing the expression of Kif5 members: Kif5a, Kif5b and Kif5c mRNAs during kidney development. Br E18.5, brain of E18.5 embryo; KY E12.5, kidney of E12.5 embryo. mRNAs of brain tissues from E18.5 embryos were used as positive controls in the real-time PCR analysis. Bars represent the means ± S.D. (n = 3). One-way ANOVA was performed to compare specific gene expression levels in the kidneys between different stages. * P<0.05 versus KY E12.5. Scale bar = 25 μm.