Electrophoretic analysis and differential sensitivity to proteolysis of PrPres in different MM2 CJD patients.
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posted on 2008-08-20, 01:21 authored by Emmanuel E. Comoy, Cristina Casalone, Nathalie Lescoutra-Etchegaray, Gianluigi Zanusso, Sophie Freire, Dominique Marcé, Frédéric Auvré, Marie-Magdeleine Ruchoux, Sergio Ferrari, Salvatore Monaco, Nicole Salès, Maria Caramelli, Philippe Leboulch, Paul Brown, Corinne I. Lasmézas, Jean-Philippe DeslysPrPres from human brain homogenates (MM2 or MV2 subtypes of sCJD) and from primate experimentally infected with BASE were purified under high concentrations of proteinase K, and detected with monoclonal antibodies that recognize either the core (3F4, Panel A) or the octapeptide region (Panel B) of the protein.
