Electron Immunomicroscopy of Pallidin in Mouse Dentate Gyrus.
Images depict immunoperoxidase electron microscopy with a pallidin monoclonal antibody in wild type (Bloc1s6+/+, A–D or Mecp2+/y, F–G), pallidin null (Bloc1s6pa/pa, E) and Mecp2 mutant hippocampi (Mecp2tm1.1Jae/y, H). A–H depict representative asymmetric axospinous synapses from the hilus of the dentate gyrus. Sp, spine; MBV, multivesicular body; CCV clathrin coated vesicle; Cist, cisterna. Structures were identified following defined ultrastructural criteria . I) Box plot depicts quantitation of pallidin immuno-positive synaptic compartments in wild type (Bloc1s6+/+ Mecp2+/y, Blue, n = 3), pallidin null (Bloc1s6pa/pa, Red, n = 1) and Mecp2 mutant tissue (Mecp2tm1.1Jae/y, Red, n = 3). Dendrite includes immuno-positive spines as well as dendritic shafts. J) Synapse count and numbers of pallidin immuno-positive synapses per genotype. Bars 200 nm.