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Electron Immunomicroscopy of Pallidin in Mouse Dentate Gyrus.

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posted on 2013-06-04, 00:20 authored by Jennifer Larimore, Pearl V. Ryder, Kun-Yong Kim, L. Alex Ambrose, Christopher Chapleau, Gaston Calfa, Christina Gross, Gary J. Bassell, Lucas Pozzo-Miller, Yoland Smith, Konrad Talbot, In-Hyun Park, Victor Faundez

Images depict immunoperoxidase electron microscopy with a pallidin monoclonal antibody in wild type (Bloc1s6+/+, A–D or Mecp2+/y, F–G), pallidin null (Bloc1s6pa/pa, E) and Mecp2 mutant hippocampi (Mecp2tm1.1Jae/y, H). A–H depict representative asymmetric axospinous synapses from the hilus of the dentate gyrus. Sp, spine; MBV, multivesicular body; CCV clathrin coated vesicle; Cist, cisterna. Structures were identified following defined ultrastructural criteria [88]. I) Box plot depicts quantitation of pallidin immuno-positive synaptic compartments in wild type (Bloc1s6+/+ Mecp2+/y, Blue, n = 3), pallidin null (Bloc1s6pa/pa, Red, n = 1) and Mecp2 mutant tissue (Mecp2tm1.1Jae/y, Red, n = 3). Dendrite includes immuno-positive spines as well as dendritic shafts. J) Synapse count and numbers of pallidin immuno-positive synapses per genotype. Bars 200 nm.