Egr-1-induced transcriptional responses in skin fibroblasts.
Dermal fibroblasts were infected with Ad-EGFP or Ad-Egr-1m (100 MOI). At the end of 24 or 48 h incubation, total RNA was isolated and subjected to genome-wide transcriptional analysis using Illumina Microarray chips (A,B) or real-time qPCR (C). A. Heatmap of differentially expressed genes (FDR<0.01 and >2- fold-change) (48 h). The color represents the fold-change of Egr-1 in comparison with the average of control sample (red = increased, green = decreased). Each row represents a probe and each column represents one sample. Genes with similar changes in expression pattern compared to the control are clustered together for 24 and 48 h. B. Comparing a subset of biological processes significantly enriched (p<0.001) with Egr-1-regulated genes at 24 and 48 h. The number in the plot indicates the differentially expressed genes belonging to individual GO categories (row) at corresponding time point (column). The total number of genes at each time point (column) is shown below the Table. The background color represents the statistical significance of a particular biological process overrepresented in the differential gene list as estimated by Hyper-geometric test. C. Real-time qPCR. Results, normalized with GAPDH mRNA, are the means ± S.D. of triplicate determinations from a representative experiment.