Efficient thymocyte development is unaffected in CD205-deficient thymus.

Thymocytes isolated from adult Ly75^−/− and Ly75^+/− littermate controls were analyzed by flow cytometry for: (A) CD4 and CD8 distribution, representative of ≥10mice, (B) Total thymus cellularity, n = ≥10, (C) CD4⁻8⁻ double negative (DN) thymocyte distribution, CD44⁺25⁻ (DN1), CD44⁺25⁺ (DN2), CD44⁻25⁺ (DN3), CD44⁻25⁻ (DN4), n = ≥4, (D) CD69⁺ CD4⁺8⁺ double positive (DP) thymocytes, n = ≥4, (E) Foxp3⁺ T regulatory CD4 thymocytes, gated on CD4⁺8⁻ TCRβ^hi CD25⁺ Foxp3⁺, n = ≥7, and (F) CD4⁺8⁻ SP4 and CD4⁻8⁺ SP ratios, n = ≥4. Statistical analysis performed using Mann Whitney U test. n.s. (not significant). Error bars show ± standard error for indicated number (n) of mice examined.