Efficient thymocyte development is unaffected in CD205-deficient thymus.
figureposted on 2013-02-19, 17:47 authored by William E. Jenkinson, Kyoko Nakamura, Andrea J. White, Eric J. Jenkinson, Graham Anderson
Thymocytes isolated from adult Ly75−/− and Ly75+/− littermate controls were analyzed by flow cytometry for: (A) CD4 and CD8 distribution, representative of ≥10mice, (B) Total thymus cellularity, n = ≥10, (C) CD4−8− double negative (DN) thymocyte distribution, CD44+25− (DN1), CD44+25+ (DN2), CD44−25+ (DN3), CD44−25− (DN4), n = ≥4, (D) CD69+ CD4+8+ double positive (DP) thymocytes, n = ≥4, (E) Foxp3+ T regulatory CD4 thymocytes, gated on CD4+8− TCRβhi CD25+ Foxp3+, n = ≥7, and (F) CD4+8− SP4 and CD4−8+ SP ratios, n = ≥4. Statistical analysis performed using Mann Whitney U test. n.s. (not significant). Error bars show ± standard error for indicated number (n) of mice examined.