Effects of the adenylyl cyclase inhibitor DDA on visual LTM formation.

(A) At 20 min prior to 8-trial visual conditioning, three groups of animals were each injected with 3 μl of saline or saline containing 100 μM or 1 mM DDA. Relative preference between the rewarded pattern and control pattern was tested before and at 1 day after conditioning. (B) At 20 min prior to 8-trial conditioning, another two groups were each injected with 3 μl of saline or saline containing 1 mM DDA. Relative preference between the rewarded pattern and control pattern was tested before and at 30 min after training. PIs for rewarded pattern before (white bars) and after (grey bars) training are shown as box plots, and the results of statistical comparison between them are indicated (** p<0.01; NS p>0.05, WCX test).