Effects of siRNA for GPR41 on SCFA-induced rise in insulin-stimulated glucose uptake in 3T3-L1 adipocytes and C2C12 myotubes.

After confirming the expression of GPR41 protein expression by transfecting with siRNA for GPR41 (siGPR41,100 nM) using Lipofectamine RNAiMAX for 48 h in 3T3-L1 adipocytes (A) or C2C12 myotubes (C), cells were treated with 300 µM propionic acid or 500 µM valeric acid for 30 min in the absence or presence of insulin (100 nM) in KRPH buffer. Glucose uptake was measured in the lysates of 3T3-L1 adipocytes (B) or C2C12 myotubes (D) as described in the Methods. Results are the means ± SEM of three similar independent experiments, each performed in quadruplicate. **P<0.01 and ***P<0.001, vs. basal glucose uptake with siControl (negative control siRNA). ⁺⁺P<0.01 and ⁺⁺⁺P<0.001 vs. basal glucose uptake with siGPR41, ^##P<0.01 vs. insulin-stimulated glucose uptake with SCFAs and siControl.