Effects of perfusion flow rate on OSB, BMMC and MMC viability.
Data presented is the response of patient sample #8 and it is representative of repeated experiments conducted using a total of 5 different patient samples (Table 1). BMMC were labeled with CFSE prior to coculture in order to detect and quantify proliferation. Three different flow rates (0.25, 0.8 and 2.5 mL/min) were tested and compared to 0 mL/min (i.e., static condition). (a) Average expansion of BMMC and CD138+ MMC was calculated at indicated flow rates, using multi-color flow cytometric analysis. (b) OSB viability in coculture was determined by flow cytometric analysis of 7-AAD staining to quantify the percentage of dead OSB among CFSE– cells. ‘OSB alone’ bar is shown as reference. (c) CD138+ MMC viability was also assessed by 7-AAD staining. * p < 0.05, ** p < 0.01.