Effects of patient-derived plasma concentration in the culture medium on OSB and MMC viability.
Data presented is the response of patient sample #10 and it is representative of repeated experiments conducted using a total of 3 different patient samples (Table 1). Patient-derived BMMC were labeled with CFSE prior to coculture in order to detect and quantify proliferation. Two different plasma concentration were tested: (1) 5% patient plasma (Pa) + 5% commercially available human AB plasma (Hu) and, (2) 10% Pa. (a) Average expansion of BMMC and CD138+ MMC were determined using multi-color flow cytometric analysis. (b) OSB viability in coculture was determined by flow cytometric analysis of 7-AAD staining to quantify the percentage of dead OSB among CFSE– cells. ‘OSB alone’ bar is shown as reference. (c) CD138+ MMC viability was also assessed by 7-AAD staining. ‘OSB alone’ bar is shown as reference. * p < 0.05, ** p < 0.01.