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Effects of mAb 130-6D treatment on pulmonary leukocyte trafficking and RSV titers.

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posted on 2013-02-19, 17:54 authored by Hayat Caidi, Jennifer L. Harcourt, Ralph A. Tripp, Larry J. Anderson, Lia M. Haynes

(A) The BAL cells from normal Ig antibody control (nIg, 300 µg/mouse) and anti-G mAb (mAb 130-6D; 300 µg/mouse) treated mice were harvested on days 3, 5, and day 7 p.i. The data are expressed as the mean number of BAL cells per ml (±standard error, SE). (B) Percent reduction in total CD4+, CD8+, RB6-8C5+, CD11b+, DX5+ and B220+ cell types after mAb 130-6D treatment relative to total cells type after nIg treatment at days 5 and 7 p.i. (C) The levels of IFN-γ cytokine production in BAL cell-free supernatant are expressed in pg/ml. (D) The level of RSV replication in lung tissue was evaluated by real-time RT-PCR (qRT-PCR) for M gene expression (relative genome level equivalent PFU/g of lung tissue x103) and infectious virus titers were determined by immunostaining plaque assay (PFU/g of lung tissue) (E). Asterisks indicate a significant difference (p<0.05) between nIg-treated and antibody treated mice. Results are representative of three independent experiments. ND indicates virus titers below the level of detection.