Effects of different inhibitory agents on 7KCh-induced cell death.

ARPE19 cells were treated with 6-15 µM 7KCh for 24 hr and the cell viability was measured by determining cellular dehydrogenase activity. (a) Viability measurements with and without 10 µM CLI095 (mean ± s.d., n = 4). (b) Viability measurements with and without 50 µg/ml LPS (mean ± s.d., n = 3). (c) Viability measurements with and without 1 µM sterculic acid (mean ± s.d., n = 4). (d) Viability measurements with and without 5 µM AG1478 (mean ± s.d., n = 3). (e) Viability measurements with and without 10 µM LY294002 (mean ± s.d., n = 3). (f) Viability measurements with and without MKP2 overexpression (mean ± s.d., n = 4). CLI095, LPS, SA, and AG1478 reduced 7KCh-induced cell death while LY294002 and MKP2 overexpression had no effect. *p<0.05, two-tailed Student's t-test.