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Effects of bDV RNA mutants on refolding of BCAII.

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posted on 07.05.2014, 03:39 by Bani Kumar Pathak, Surojit Mondal, Amar Nath Ghosh, Chandana Barat

A) Bar diagram shows percent aggregation reduction during BCA II-m refolding by bDV RNA, mDV RNA and bDV RNA mutants U2585C (UC), delG2252 (delG). The turbidities in each case were measured at 320 nm, 12 minutes after return to refolding conditions and turbidity in absence of chaperone was assumed as 100%. B) Comparison of reactivation yields of BCAII-m (0.3 µM) after 30 min of refolding in absence of the chaperone (Self) and in presence of bDV RNA1(R1), bDV RNA1+RNA2 (R1+R2), RNA1+3% Ethanol (R1+EtOH), del G2252 bDV RNA (delG), del G2252+ RNA2 (delG+R2), U2585C (UC), U2585C bDV RNA+RNA2 (UC+R2) and U2585C bDV RNA+3% Ethanol (UC+EtOH). C) Comparison of the reactivation yields of BCAII-m (0.9 µM) after 30 min of refolding in absence of the chaperone (Self), in presence of del G2252 bDV RNA and mDV RNA. BCAII-m reactivation upon addition of RNA 2 portion of bDV at zero minute (mDV+R2 0′and delG+R2 0′) and after ten minutes of initiation of refolding (mDV+R2 10′ and delG+R2 10′) are also shown.

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