Effects of ammonia exposure on total Na+/K+-ATPase (Nka) protein abundance.
Protein abundance of Nka, based on the α5 anti-NKA antibody which is known to react with all Nka/NKA α-isoforms, in the brain of Monopterus albus kept in freshwater (FW; control) or exposed to 50 mmol l−1 NH4Cl for 1 day or 6 days. (A) An example of the immunoblots of Nka and actin. (B) The intensity of the Nka band normalized with respect to that of actin. Results represent mean+S.E.M. (N = 3). Means not sharing the same letter are significantly different (P<0.05).