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Effects of ammonia exposure on total Na+/K+-ATPase (Nka) protein abundance.

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posted on 31.12.2013, 04:48 by Xiu L. Chen, Nicklaus L. J. E. Wee, Kum C. Hiong, Jasmine L. Y. Ong, You R. Chng, Biyun Ching, Wai P. Wong, Shit F. Chew, Yuen K. Ip

Protein abundance of Nka, based on the α5 anti-NKA antibody which is known to react with all Nka/NKA α-isoforms, in the brain of Monopterus albus kept in freshwater (FW; control) or exposed to 50 mmol l−1 NH4Cl for 1 day or 6 days. (A) An example of the immunoblots of Nka and actin. (B) The intensity of the Nka band normalized with respect to that of actin. Results represent mean+S.E.M. (N = 3). Means not sharing the same letter are significantly different (P<0.05).