Effects of ammonia exposure on Na+/K+-ATPase α3 (Nkaα3) protein abundance.
Protein abundance of Nkaα3, based on anti-NKAα3 antibody, in the brain of Monopterus albus kept in freshwater (FW; control) or exposed to 50 mmol l−1 NH4Cl for 1 day or 6 days. (A) An example of the immunoblots of Nkaα3 and actin. (B) The intensity of the Nka band normalized with respect to that of actin. Results represent mean+S.E.M. (N = 3). Means not sharing the same letter are significantly different (P<0.05).