Effects of Ure2 aggregates on the DKOR cell line.

(A), (B) Immunofluorescence co-labeling for clathrin and Ure2 in the DKOR cell line at 48 h after extracellular addition of 3 µM different states of Ure2. Green fluorescence is clathrin, red is Ure2 and blue is Hoechst 33258 nuclear stain (bar = 12 µm). (A) DKOR cells with expression of clathrin. (B) DKOR cells without expression of clathrin. (C), (D) Growth curves of the DKOR cell line in the presence of 3 µM Ure2 or its amyloid aggregates. The error bars represent the S.E. of the mean of three independent measurements. (C) DKOR cells with expression of clathrin. (D) DKOR cells without expression of clathrin.