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Effects of IL-6 inhibition on aggressive tumor behavior and EMT changes.

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posted on 30.04.2013, 01:19 by Miao-Fen Chen, Paul-Yang Lin, Ching-Fang Wu, Wen-Cheng Chen, Chun-Te Wu

A. The invasive capacity of bladder cancer cells with or without the IL-6 silencing vector was evaluated by migration scratch assays. The results from representative slides are shown. Column, mean of three separate experiments. Bars, SD. *, P<0.05. B. The invasive capacity of bladder cancer cells with or without the IL-6 silencing vector was evaluated by murine orthotopic tumor implantation. The representative slides and quantitative data are shown. The y-axis represents the ratio of mice presenting intravesicular tumors, normalized to that received orthotopic tumor implantation. The IL-6 silencing vector decreased the rate of tumor implantation in the bladder and was associated with a smaller tumor size. Column, mean of three separate experiments. Bars, SD. *, P<0.05. (CV, cells transfected with the control vector; IL-6 SV, cells transfected with the IL-6 silencing vector). C. Change in E-cadherin in cells was evaluated and the representative micrographs are shown, with the respective immunofluorescent colors (DAPI, blue; GFP, green; E-cadherin, red). D. Change in EMT-associated proteins in transfectants was evaluated by Western blotting analysis (CV, cells transfected with the control vector; IL-6 SV, cells transfected with the IL-6 silencing vector). E. Change in EMT-associated proteins in cells treated with JAK inhibitor- AG490.

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