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Effects of BCAA supplementation on liver fibrosis in rats after 16 weeks of DEN administration.

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posted on 01.11.2013, 03:14 authored by Jung Hoon Cha, Si Hyun Bae, Hye Lim Kim, Na Ri Park, Eun Suk Choi, Eun Sun Jung, Jong Young Choi, Seung Kew Yoon

The relative band intensities of the fibrosis markers are shown in the right-hand panels (A). The expression levels of mRNA for TGF-β1, Col1α2, Col3α1, Smad-4, and TIMP-2 were determined by quantitative Real-Time reverse-transcription polymerase chain reaction (RT-PCR) (B). TIMP-2 protein expression was determined by Western blotting (C). The level of expression of each gene is normalized to those of GAPDH (A and B) and β-actin (C). The lanes contain the mRNA (A) and protein (C) samples from three rats per group. Values shown are means ± standard deviation. *P<0.05.