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Effects of 13-oxo-ODA on mRNA expression levels of genes involved in lipid metabolism in the liver of HFD-fed KK-Ay mice.

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posted on 2012-02-09, 01:25 authored by Young-il Kim, Shizuka Hirai, Tsuyoshi Goto, Chie Ohyane, Haruya Takahashi, Taneaki Tsugane, Chiaki Konishi, Takashi Fujii, Shuji Inai, Yoko Iijima, Koh Aoki, Daisuke Shibata, Nobuyuki Takahashi, Teruo Kawada

The mRNA expression levels of genes involved in fatty acid metabolism (CPT1a, AOX, FAT, ACS, and UCP2; Fig. 3A) and in TG synthesis (SREBP1c, ABCA1, ABCG1, and FAS; Fig. 3B) in the liver of control and 13-oxo-ODA-fed mice were quantified by real-time PCR. The relative amount of each transcript was normalized to the amount of the 36B4 transcript. The expression levels in the vehicle control are set at 100% and the relative expression levels are presented as fold induction with respect to that in the vehicle control. (C) Protein expression of AOX and β-actin in the liver of control and 13-oxo-ODA-fed mice were compared by immunoblotting assay. The same amounts of protein (25 µg/lane) were loaded and blotted onto PVDF membranes. The membranes were sequentially treated with primary antibodies as indicated and secondary antibodies conjugated with HRP. The enhanced chemiluminescence system was used for visualization of membranes. Data are presented as mean ± SEM (n = 6−8). *; p<0.05 versus control.

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