Effect of vitamin C on ethanol-induced CYP2A6 expression level and CYP2E1-mediated oxidative stress.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
(A) CYP2A6 mRNA. (B) CYP2A6 protein. Both mRNA and protein expression levels were determined at 12 h in the presence of 100 mM ethanol treatment (−/+100 µM vitamin C 1 h prior to ethanol treatment). Blots are representative of at least three replicates. For each experiment, the mRNA/protein levels of various treatments were normalized relative to the untreated control, which has been set to 100%. (C) Representative figure of the determination of ROS levels at 2 h ethanol treatment with 0 and 100 µM vitamin C pretreatment for 1 h. The events (cell population) are presented in Y-axis and relative fluorescence intensity is presented in X-axis. (D) Bar graphs of MFI in the presence and absence of ethanol and vitamin C. The mean ± SD was calculated from at least triplicates and significance (p≤0.05; *) was determined using one-way ANOVA. The experiment was repeated three times.