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Effect of sortilin on APP distribution in lipid rafts.

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posted on 21.05.2013, 01:30 by Miao Yang, Balaji Virassamy, Swarna Lekha Vijayaraj, Yoon Lim, Khalil Saadipour, Yan-Jiang Wang, Yan-Chuang Han, Jin-Hua Zhong, Carlos R. Morales, Xin-Fu Zhou

A: HEK293 cells co-transfected with APP695 and sortilin constructs were lysed in Triton-X-100 buffer and subjected to discontinuous sucrose density gradient ultracentrifugation fractionation. Equal volumes from each fraction were examined by WB for APP and flotillin-1. B: Lack of sortilin increases APP distribution within lipid rafts in sortilin KO mice. Mouse brains were homogenized in Triton-X-100 buffer and subjected to discontinuous sucrose density gradient ultracentrifugation fractionation. Equal volumes from each fraction were examined by WB for APP and flotillin-1. Bars represent mean± SEM (n = 3) from three independent experiments. The star (*) indicates p<0.01.

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