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Effect of salinomycin and recombinant TRAIL on cell growth (A), cell death (B) and TRAIL-R2 (C) expression of GSC neurospheres.

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posted on 16.04.2014, 02:46 by Alessia Calzolari, Ernestina Saulle, Maria Laura De Angelis, Luca Pasquini, Alessandra Boe, Federica Pelacchi, Lucia Ricci-Vitiani, Marta Baiocchi, Ugo Testa

A and B – Glioblastoma neurosphere clones GSC1, GSC30 and GSC83 were grown for 48 hours either in the absence (C) or in the presence of either TRAIL (10 ng/ml) or Salinomycin 1 µM or Salinomycin 5 µM or Salinomycin 1 µM+TRAIL or Salinomycin 5 µM+TRAIL and the number of viable cells was determined by the quantification of cellular ATP content using the Cell Titer-Glo Luminescen Cell Viability Assay Kit (A) and the percentage of apoptotic cells by the Annexin-V binding assay (B). The results represent the mean values observed ± SEM observed in three separate experiments, each performed in duplicate. For all these treatments and for all the three neurosphere clones, the difference between the values observed for TRAIL and Salinomycin 1 µM+TRAIL or Salinomycin 5 µM+TRAIL were statistically significant (p = <0.05 or <0.01) and the values observed for Salinomycin 1 µM and Salinomycin 1 µM+TRAIL or Salinomycin 5 µM and Salinomycin 5 µM+TRAIL (p = <0.05 or <0.01) were statistically significant. (C) Flow cytometric detection of TRAIL-R2 expression in GSC1 cells grown for 24 h either in the absence or in the presence of salinomycin (1 or 5 µM). The results are expressed in terms of mean fluorescence intensity (MFI) values observed in three separate experiments (mean values±SEM). The differences between the values observed between 1 µM or 5 µM salinomycin and control are statistically significant (both p<0.01).

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