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Effect of receptor trafficking on synaptic transmission.

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posted on 03.10.2011, 02:43 by Dominik Freche, Ulrike Pannasch, Nathalie Rouach, David Holcman

(A) For a PSD (diameter 200 nm with 100 AMPARs marked blue at time 0) and an outside reservoir (diameter 400 nm with 300 AMPARs marked pink at time 0), the time course of AMPAR exchange by receptor diffusion () is shown. Within 50 ms the two AMPAR populations (blue and pink lines) are equilibrate to 75%, while the average number of AMPARs on the PSD remains constant. Error bars: variance, light colors: sample trajectories. (B) The time course of exchange is shown for a PSD with partially impenetrable boundary. Despite placing 10 equally-spaced barriers (indicated in the inset by the dashed circle) covering 0 (blue) to 90% (green) of the total PSD boundary, the mean number of receptors (red) inside the PSD does not change. (C, E, G) Stimulation with two consecutive pulses (frequency ranging from 20 Hz–0.4 Hz), each leading to the release of 1 vesicle in the center of the AZ in either presence or absence of AMPAR diffusion. The paired-pulse ratio (PPR) is shown at the maximal number of open AMPARs for the JS (C), MN (E) and RL model (G). The effect of AMPAR diffusion on the PPR was maximal for 20 ms. (D, F, H) During 10 pulses of a 20 Hz pulse train, the number of diffusing (green) and immobile (red) open AMPARs decays, while the number of desensitized AMPARs increases (dashed).

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