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Effect of proteasomal inhibitor on B6 and BTBR allele of b-tomosyn-2.

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posted on 06.10.2011, 02:03 by Sushant Bhatnagar, Angie T. Oler, Mary E. Rabaglia, Donald S. Stapleton, Kathryn L. Schueler, Nathan A. Truchan, Sara L. Worzella, Jonathan P. Stoehr, Susanne M. Clee, Brian S. Yandell, Mark P. Keller, Debbie C. Thurmond, Alan D. Attie

A) The mammalian expression plasmid containing the mock, b-tomosyn-2 (Serine912)-myc or b-tomosyn-2 (Leucine-912)-myc were transfected in HEK293Tcells. After 16 h incubation in DMEM supplemental media, cells expressing b-tomosyn-2-myc were treated with MG132 (100 µM) or DMSO for 6 h. Equal amounts of protein were subjected to western blot analysis and the level of b-tomosyn-2-myc protein was determined using anti-myc antibody. The actin was used as a loading control. B) Shows the quantitation of the signals for three experiments. Values are means ± S.E. of N = 3. *p≤0.05 for the expression of b-tomosyn-2-myc in the samples treated with MG132 vs. DMSO.