Effect of osmotic shock on the keratin cytoskeleton in the NEB-1 cell line (A) and the EBS derived cell line KEB-7 (B).
figure
posted on 2012-02-21, 02:16 authored by Daniel R. Beriault, Oualid Haddad, John V. McCuaig, Zachary J. Robinson, David Russell, E. Birgitte Lane, Douglas S. FudgeBoth cell lines were subjected to hypo-osmotic shock, then fixed and permeabilized and stained for K14 intermediate filaments. Clear peripheral aggregates were seen in KEB-7 cells 30 min after osmotic shock. No filament fragmentation or aggregates were observed for NEB-1 cells. Scale bar = 22 µm.
