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Effect of mimosine and CPX on deoxyhypusine hydroxylase activity.

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posted on 16.03.2012, 01:59 by Bhavna Chawla, Ravi Ranjan Kumar, Nidhi Tyagi, Gowri Subramanian, N. Srinivasan, Myung Hee Park, Rentala Madhubala

Recombinant LdDOHH (5 µg) was incubated with either (A) mimosine (5, 10 or 20 µM) or (B) CPX (5, 10 and 15 µM) for 10 min at 37°C. Recombinant hDOHH was incubated with varying concentrations of (C) mimosine (20, 40, 60, 75 and 100 µM) and (D) CPX (15, 30, 45, 75 and 100 µM) for 10 min at 37°C. [3H]Hypusine formation was then measured as detailed in the Methods section. Results are mean ± SD of triplicate samples. *, p<0.05; **, p<0.002 and ns indicates not significant (p>0.05).

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