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Effect of miR-122 overexpression on gene expression in hESC.

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posted on 18.11.2008, 01:56 by Galit Tzur, Asaf Levy, Eti Meiri, Omer Barad, Yael Spector, Zvi Bentwich, Lina Mizrahi, Mark Katzenellenbogen, Etti Ben-Shushan, Benjamin E. Reubinoff, Eithan Galun

QRT-PCR results of gene expression in 14d-differentiated HES2 cells expressing mutant (mut) or wt miR-122. The reactions were performed on the same RNA samples that were analyzed on the microarrays. Results are expressed as relative quantification of the gene level in each sample relative to undifferentiated cells transduced with the relevant miRNA (wt or mutant), and normalized to GUSB. Each reaction was performed in triplicates and the average RQ of 3 independent experiments is shown with the SD and the p value between mut diff and wt diff cells when significant.


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