Effect of miR-107 on CDK5R1 mRNA in polysomal and subpolysomal lysates.
qRT-PCR of CDK5R1 mRNA in polysomal and subpolysomal fractions. SK-N-BE cells were transfected with 100 nM pre-miR-107 or anti-miR-107 for 48 h, then the mRNA–ribosomal complexes were separated through sucrose gradient centrifugation. Expression is normalized on the housekeeping Alu transcript. The percentages of the transcript obtained from the two fractions are calculated on the sum of the 2−ΔCt of the mRNA from polysomal and subpolysomal compartments, for pre-miR-107, anti-miR-107 treatments and the control (lipofectamine).