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Effect of cumanin, cordilin and daucosterol against T. cruzi trypomastigotes.

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posted on 10.10.2013, 07:44 by Valeria P. Sülsen, Silvia I. Cazorla, Fernanda M. Frank, Laura C. Laurella, Liliana V. Muschietti, Cesar A. Catalán, Virginia S. Martino, Emilio L. Malchiodi

Bloodstream trypomastigotes diluted at 1.5×106/ml in complete liver infusion tryptose medium, were seeded by duplicate in the presence of different concentration of each compound (0–376 µM, 0–375 µM and 0–174 µM, for cumanin, cordilin and daucosterol, respectively) and incubated at 4°C for 24 h. The remaining live parasites of (A) RA strain, and (B) K98 strain, in each sample was determined in 5 µl of cell suspension diluted 1/5 in lysis buffer (0.75% NH4Cl, 0.2% Tris, pH 7.2) and counted in a Neubauer chamber. Values represent mean ± SEM from three independent experiments carried out in duplicate.