Effect of anti-E mAb 4G2 on the infectious properties of immature WNV particles in vitro and in vivo.
(A) P388D1 cells were infected with immature WNV opsonized with increasing concentrations of 4G2 at MOG 10. At 26 hpi, the supernatant was harvested and virus production was analyzed by plaque assay on BHK21-15 cells. Data are expressed as means of at least two independent experiments performed in duplicate. The error bars represent standard deviations (SD); (n.d.) denotes “not detectable”. Student's t-tests were used to determine significance; *, P<0.01. (B) Immature WNV was incubated with different concentrations of anti-E 4G2 for 1 hr at 37°C, and injected in mice. A total of 3.4×107 GCPs were given per mouse. Five mice were used for each experimental condition.