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Effect of Viscum album on the stability of COX-2 protein as analyzed by flow cytometry.

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posted on 2015-02-09, 02:56 authored by Chaitrali Saha, Pushpa Hegde, Alain Friboulet, Jagadeesh Bayry, Srinivas V. Kaveri

A549 cells were stimulated with IL-1β for 90 minutes with or without VA Qu Spez. Cells were harvested at different time intervals after blocking the protein synthesis with cyclohexamide (10 μg/ml) for 90 minutes till 11 hours. Normalised percentage COX-2 expression as measured in intracellular staining by flow cytometry (A) and mean fluorescence intensity (MFI) of COX-2 expression (B) is shown. Data is representative of mean ±SEM of three independent experiments.