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Effect of PMBPs on NFκB activity and MAPK pathways.

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posted on 05.11.2015 by Jia Liu, Jing Bai, Guoqiang Jiang, Xinli Li, Jing Wang, Dachang Wu, Lawrence Owusu, Ershao Zhang, Weiling Li

(A,B) The cells were treated with PMBPs (0, 5, 10, 25 μg/ml) for 24 h. Cell lysates were prepared and subjected to Western blot analysis. The protein level of nuclear p65, p-IκBα, IκBα, p-ERK, p-p38 and p-JNK were measured. (C,D) Histograms show mean (±SD) level of nuclear p65, p-IκBα, IκBα, p-ERK, p-p38 and p-JNK from three independent experiments. The nuclear p65, p-IκBα, IκBα, p-ERK, p-p38 and p-JNK expression levels were expressed relative to loading control β-Actin and standardized to the non-treated control group. *P<0.05 and **P<0.01 compared to the control group.

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