Effect of LRRK2 expression on dopamine (DA+3-MT) extracellular level.
PC12-derived cell lines were left untreated (A) or treated (C) for 48 h with 0.2 µg/mL doxycycline. After 60 minutes of stabilization, three baseline dialysates were collected at 20-minute intervals [gray bars in panel (A), and gray bars with stripes in panel (C)]. Starting from 60 minutes, nicotine was infused for 60 minutes (capped lines). Microdialysates were continuously recovered during drug infusion and after nicotine discontinuation [black bars in panel (A), and dotted bars in panel (C)]. Values are mean ± SEM and refer to dopamine concentrations in dialysates. Statistical significance was assessed using analysis of variance (ANOVA) for differences over time determined by Newman-Keules t test and unpaired t-tests. *p<0.05 compared with pertinent baseline values of all groups. Graphs in panel (B) and (D) represent the area under curve (AUC) values. (B) Basal dopamine concentration in dialysates of PC12 cell lines untreated (gray bars corresponding to the same color in panel A) or treated with 0.2 µg/mL doxycycline [gray bars with stripes corresponding to same color in panel (C)]. (D) Dopamine concentrations in dialysates integrated after nicotine administration in PC12 cell lines untreated [black bars corresponding to same color in panel (A)] or treated with 0.2 µg/mL of doxycycline [dotted bars corresponding to same color in panel (C)]. AUC values are mean ± SEM. *p<0.05 vs corresponding control.