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Effect of ICV olanzapine and AICAR administration on phosphorylation of hypothalamic AMPK and ACC.

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posted on 20.02.2013, 17:38 by Johan Fernø, Luis Varela, Silje Skrede, María Jesús Vázquez, Rubén Nogueiras, Carlos Diéguez, Antonio Vidal-Puig, Vidar M. Steen, Miguel López

Western blot analysis of hypothalamic pAMPK and pACC from rats sacrificed 30 minutes after ICV injection of a) olanzapine or b) AICAR, relative to control rats (DMSO). Calculations are based on results from 6 rats for each treatment group, run in duplicate. Representative images for the calculated difference were selected. Each lane (pACC, pAMPK and β-actin) always represents results on the same gel from the same rat. * P≤0.05 vs. vehicle. ** P≤0.01 vs. vehicle. *** P≤0.001 vs. vehicle.