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Effect of ATP on the thermal deactivation prevention ability of M. leprae HSP18.

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posted on 26.03.2015 by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas

The enzyme activity of MDH was measured in absence and presence of 30 μM HSP18 preincubated with/without (A) 0.01–3 mM ATP; (B) 1 mM ATP/ATP-γS during its thermal denaturation at 43°C. The assay also performed with 30 μM BSA alone or preincubated with 0.01–3 mM ATP. ***p<0.0005.