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Effect of ATP on the surface hydrophobicity of M. leprae HSP18.

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posted on 26.03.2015 by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas

First, HSP18 was incubated in absence (solution 1) and presence of presence of 0.01 mM ATP (solution 6)/1 mM ATP (solution 2)/1 mM ATP-γS (solution 5) at 25°C for 1 hr. Then, part of solution 1 and 2 were dialysed overnight against 50 mM phosphate buffer, pH 7.5. The resultant solution achieved from the dialysis of solution 1 and 2 were termed as solution 3 and 4, respectively. All these solutions were incubated with 10 μM bis-ANS at 25°C for 1 hr. The protein concentration was 0.05 mg/ml. The fluorescence spectrum of bis-ANS bound to different samples was recorded in the range 450–550 nm at 25°C. The excitation wavelength was 390 nm. Trace 1: bis-ANS + solution 1; Trace 2: bis-ANS + solution 6; Trace 3: bis-ANS + solution 2; Trace 4: bis-ANS + solution 5; Trace 5: bis-ANS + solution 3; Trace 6: bis-ANS + solution 4.

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