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Effect of ATP on the structure/conformation of M. leprae HSP18.

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posted on 26.03.2015 by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas

(A) Intrinsic tryptophan fluorescence spectra of HSP18 (0.05 mg/ml) in absence and presence of 1 mM ATP were recorded in the range 310–400 nm at 25°C. The excitation wavelength was 295 nm. Excitation and emission slit widths were 5 nm each. Data were recorded at a wavelength resolution of 0.5 nm. (B) Far-UV CD spectra of HSP18 in absence and presence of 1 mM ATP. Spectra were recorded for 0.2 mg/ml protein (in 10 mM phosphate buffer, pH 7.5) using a cell of 1 mm path length. The data interval was 1 nm. (C) Intensity particle size distribution spectra of M. leprae HSP18 were recorded at 25°C temperature in presence or absence of 1 mM ATP. Each of these spectra is an average of 48 scans.