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Effect of APOBEC3s on ΔVif HIV-1 DNA synthesis.

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posted on 20.02.2013, 04:35 by Silvia Sanchez-Martinez, Amanda L. Aloia, Demetria Harvin, Jane Mirro, Robert J. Gorelick, Patric Jern, John M. Coffin, Alan Rein

293-mCAT1 cells were infected with ΔVif HIV-1. Infectivity and RT activity were assayed as described [25], [29], [30]. Twenty-four hours after infection, the cells were lysed and assayed by real-time PCR for Luciferase DNA (black and green lines) as described in Materials and Methods [32]. Specific infectivity is represented with red and blue lines.

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