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Effect of ABCG2 overexpression on the expression of SLC transporter genes and MPP, glucose and glutamine transport.

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posted on 04.11.2015, 06:20 by Maricla Galetti, Pier Giorgio Petronini, Claudia Fumarola, Daniele Cretella, Silvia La Monica, Mara Bonelli, Andrea Cavazzoni, Francesca Saccani, Cristina Caffarra, Roberta Andreoli, Antonio Mutti, Marcello Tiseo, Andrea Ardizzoni, Roberta R. Alfieri

Down-regulation of SLC transporter expression (SLC22A1, SLC22A3, SLC2A1, SLC38A2, SLC3A2, SLC7A5, and SLC7A6) in HEK293/R2 cells versus HEK293 was evaluated by PCR drug transporter array (A). Fold decrease expression was determined by the ΔΔCT method normalized to five housekeeping genes. Values given are the means (± SD) of three independent experiments (*P<0.05; **P<0.01). Initial velocity (5 min) of [3H]MPP (B), [3H]2-deoxy-D-glucose (C) and [3H]Glutamine (D) uptake was determined and values given are the means (± SD) of three independent determinations (*P<0.05; **P<0.01).