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Ear-tissue can be visualized at the cellular level in living animal.

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posted on 02.01.2013 by Benny J. Chen, Yiqun Jiao, Ping Zhang, Albert Y. Sun, Geoffrey S. Pitt, Divino Deoliveira, Nicholas Drago, Tong Ye, Chen Liu, Nelson J. Chao

(a) This picture showing the location of an engrafted heart graft in the ear pinna. (b) Skeletal muscle and small intestine tissues from EGFP mice were subcutaneously transplanted into the ear pinnae of BALB/c nude mice. The images were obtained 9 (muscle) and 22 (intestine) weeks after transplantation. The images were taken 107 µm (muscle) deep from surface. The depth for intestine could not be determined. (c) Heart and kidney tissues from EGFP mice were subcutaneously transplanted into the ear pinnae of BALB/c nude mice. Rhodomin B conjugated dextran was injected i.v. to visualize the blood vessels. The images were obtained 9 (heart) and 2 (kidney) weeks after transplantation. Green = EGFP; red = dextran. (d) EGFP+ neonatal thymic tissue was subcutaneously transplanted into the ear pinnae of BALB/c nude mice. Eight weeks later, the mice were irradiated and transplanted with DsRed+ T cell depleted bone marrow cells. The image was taken 4 weeks after bone marrow transplantation at the depth of 103 µm. Green = EGFP; red = DsRed. Scale bar = 100 µm.

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