E-cadherin mis-expression in the cortical zone upregulates Patched expression and perturbs zonal boundaries.
(A–E) Mis-expression of E-cadherin (Ecad) in cortical zone hemocytes expands the Ptc expression domain, but has no effect on plasmatocyte differentiation. Eater-Gal4 females were crossed to (A,C) control (+) or (B,D) UAS-Ecad males. (B) Eater-Gal4 driven Ecad significantly increased the Ptc expression domain compared to (A) the control. (C,D) However, Eater-Gal4 driven Ecad had no effect on plasmatocyte differentiation. Plasmatocytes were identified using the cell-specific marker, P1. Yellow dotted lines delineate the entire lymph gland; white dotted lines delineate the Ptc expression domain. Scale bars: 50 µm. (E) Histogram showing the percentage of Ptc labeled cells per primary lymph gland lobe in Eater-Gal4 or hml-Gal4 driven Ecad lymph glands compared to controls (+). Two tailed Student’s t-test; error bars show standard deviation; P values are as shown; n = 12. (F–G”) hml-Gal4 driven Ecad expression produces an increase in the following: 1) the Ptc expression domain; 2) the number of cells that are positive for both Ptc and hml-Gal4 driven GFP (Ptc+; hml>GFP+); and 3) the number of hml>GFP+ hemocytes within the medial region of the lymph gland. (F,G) Lymph glands co-stained for Ptc and GFP. (F’,F”,G’,G”) The same lymph glands showing only (F’,G’) Ptc staining and (F”,G”) GFP staining. (F–G”) Insets are an enlarged region of medullary zone showing co-expression of Ptc and GFP. (H–I”) Eater-Gal4 driven Ecad expression has no effect on Odd or Pxn expression, but produces an increase in the number of Pxn+ hemocytes within the medial region of the lymph gland. (H,I) Lymph glands co-stained for Odd and Pxn. (H’,H”,I’,I”) The same lymph glands showing only (H’,I’) Odd staining and (H”,I”) Pxn staining. (H–I”) Insets are an enlarged region showing expression of Pxn+ hemocytes or Odd+ prohemocytes. Scale bars: 50 µm.