figshare
Browse
Figure_4.tif (236.86 kB)

EGF treatment but not silencing of REDD1 provides a partial protective effect against TAIII cytotoxicity.

Download (0 kB)
figure
posted on 2009-09-30, 00:40 authored by Frank W. King, Sylvia Fong, Chandi Griffin, Mark Shoemaker, Rick Staub, Yan-Ling Zhang, Isaac Cohen, Emma Shtivelman

A. Levels of REDD1 and phosphorylation of s6 protein in MDAMB231 cells transduced with control (231-vec) or REDD1 siRNA expressing lentivirus (231-REDD1KD). Cell extracts were analyzed by Western blotting before and 4 hours after treatment with 5 µM of TAIII. B. Effect of addition of exogenous EGF on survival of BT474 and MDAMB231 cells treated with TAIII for 24 hours (4 µM for BT474 and 7.5 µM for MM231). C. Effect of exogenous EGF on Akt activity, mTORC1 activity and mTOR phosphorylation in cells treated with TAIII for 16 hours. Western blotting was done with the phospho-specific antibodies and antibodies detecting total levels of the indicated proteins.

History

Usage metrics

    PLOS ONE

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC