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EGCG induces H2O2 formation.

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posted on 21.08.2014, 04:22 by Job D. F. Inacio, Luiza Gervazoni, Marilene M. Canto-Cavalheiro, Elmo E. Almeida-Amaral

Leishmania braziliensis was cultivated in Schneider's Drosophila medium at 26°C as for 72 h in the absence or presence of EGCG (62.5–500 µM). H2O2 was measured using Amplex red as described in the Materials and Methods (Panel A). The data are expressed as the fold increase in H2O2 production relative to the control. The values presented are the mean ± standard error of three different experiments. * indicates a significant difference relative to the control group (p<0.05); ** indicates a significant difference relative to the control group (p<0.01). Panel B: Correlation between the H2O2 production and inhibition of L. braziliensis viability by EGCG (R2 = 0.975).

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