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EB1 antibody injection induces anaphase-specific cortical blebbing.

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posted on 21.12.2011, 00:10 by Anke Brüning-Richardson, Kelly J. Langford, Peter Ruane, Tracy Lee, Jon M. Askham, Ewan E. Morrison

A: Stills from a recording of a representative cell microinjected with 1A11, showing the appearance of cortical blebbing during anaphase (arrows). Scale bar = 10 µM. B: The percentage of cells displaying cortical blebbing is first shown as a percentage of the total number of microinjected cells examined (including those that failed to complete mitosis) and then as a percentage of microinjected cells that successfully completed mitosis. In both cases a significantly higher proportion of cells microinjected with antibody 1A11 displayed blebbing (p<0.005 and p<0.05). 1A11-induced blebbing was only seen during anaphase whereas a lower level of blebbing was noted throughout mitosis in cells injected with anti-APC antibodies (see Figure 2 panels D and E for examples of blebbing in metaphase cells injected with anti-APC antibodies). Blebbing was not observed in mitotic cells injected with the non-specific IgG 4 U (zero entry in first column). Significant results are indicated by an asterisk. (4 U, n = 16 for total number of microinjected cells, n = 12 for cells with completed mitosis; 1A11, n = 19 and 11; ALI 12–28 n = 16 and 9; C-APC 9.9, n = 18 and 16; C-APC 28.9, n = 15 and 11).

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