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Downregulation of N-cadherin expression by hFOB1.19 OSB using shRNA lentiviral contsructs.

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posted on 14.05.2015, 02:50 by Wenting Zhang, Yexin Gu, Qiaoling Sun, David S. Siegel, Peter Tolias, Zheng Yang, Woo Y. Lee, Jenny Zilberberg

(a) Dot plot representation of flow cytometric data showing reduced CD325 staining in shRNA-tranduced hFOB1.19 cells. GFPhigh cells (42.92%) were CD325-, 8.54% of the GFPlow cells still expressed N-cadherin, and 34.7% of transduced cells remained GFP-CD325+. (b) N-cadherin protein was also determined by Western blot analysis. Cell lysates were immunoblotted using monoclonal antibodies against N-cadherin and actin. Density of the bands of mock-transduced and N-cadherin knockdown hFOB1.19 of one representative experiment are shown.

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