Downregulation of N-cadherin expression by hFOB1.19 OSB using shRNA lentiviral contsructs.
(a) Dot plot representation of flow cytometric data showing reduced CD325 staining in shRNA-tranduced hFOB1.19 cells. GFPhigh cells (42.92%) were CD325-, 8.54% of the GFPlow cells still expressed N-cadherin, and 34.7% of transduced cells remained GFP-CD325+. (b) N-cadherin protein was also determined by Western blot analysis. Cell lysates were immunoblotted using monoclonal antibodies against N-cadherin and actin. Density of the bands of mock-transduced and N-cadherin knockdown hFOB1.19 of one representative experiment are shown.