Dose-response analyses in AIZ-AR cells after treatment with steroid compounds—antagonist mode with dihydrotestosterone.
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Cells were treated for 24 h with various endogenous and synthetic steroids in the presence of 5α-dihydrotestosterone (DHT; 100 nM). Cells were lysed and luciferase activity was measured. Data are mean of triplicate measurements and are expressed as a fold induction over DMSO-treated cells. Similar data were obtained from three consecutive cell passages. Upper plots—androgens, middle plots—corticoids, lower plots—gestagens and estrogens.