Donor DLI-derived T cell expansion following the depletion of donor BM-derived T cells.
MC preparation and DLI administration were performed as detailed in the legend to Figure 4, and treated with anti-Thy1.2 or Rat IgG starting at day 10 post-DLI. MCs were sacrificed 5 (n = 3 per group) and 20 (Rat IgG, n = 3; αThy1.2, n = 4) days after antibody treatment (i.e., 15 and 30 days after DLI, respectively), and the levels of Th1.1+ and Thy1.2+ T cells in various tissues were determined by flow cytometry using anti-Thy1.1 mAb. (A) Representative staining profiles (Left) and levels (mean±SEM) of Thy1.2+ (i.e., cells stained negative for Thy1.1) T cells in various tissues at day 5 post-antibody treatment (15 day after DLI). (B,C) Numbers (mean±SEM) of total (B) and DLI-derived Thy1.1+ (C) T cells (CD8 and CD4 T cells are shown at the top and bottom panels, respectively) in WBC, spleen and liver at the indicated time points. *, p<0.05; **, p<0.01; ***, p<0.005.